Étude comparative du genre Kineosporia fondée sur des approches moléculaires et chimiotaxonomiques utilisant les coefficients de Jaccard et d’Ochiai

Abstract

This research is based on a two-pronged methodological approach aimed at studying eight species of the genus Kineosporia (phylum Actinomycetota): K. aurantiaca, K. succinea, K. rhizophila, K. mikuniensis, K. rhammosa, K. babensis, K. mesophila, and K. corallincola. On the one hand, a chemotaxonomic analysis was conducted by applying Jaccard and Ochiai similarity coefficients to the chemical composition data of the cell walls. On the other hand, a molecular study was conducted based on 16S rRNA gene sequencing, coupled with bioinformatics algorithms for sequence alignment and genetic distance estimation. The main objective was to identify the most closely related species to Kineosporia corallincola (used as a reference) and to compare the results obtained by these two methods. The results revealed a partial divergence: the chemotaxonomic approach identifies K. aurantiaca as the species closest to K. corallincola, while the molecular analysis identifies K. babensis as the most closely related. This study thus highlights the complementary nature of chemotaxonomic and molecular approaches for reliable taxonomic classification.